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PDA10
Proteomics Data AnalysisCourse Timetable (provisional) |
| PDA10 | Proteomics Data Analysis |
| Mon, Sept 13th | Origins of the Data
Day #1
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| 09:30 - 11:00 |
Introductory remarks Mass spectrometers: function and types |
| 11:00 - 11:30 | Coffee Break |
| 11:30 - 12:30 | Practical: a first look at mass spectral data (quality control) |
| 12:30 - 14:00 | Lunch Break |
| 14:00 - 16:00 | Gel-free proteomics strategies and methodologies |
| 16:00 - 16:30 | Tea Break |
| 16:30 - 18:00 | Practical: continued look at mass spectral data (clustering) |
| Tue, Sept 14th | Search engines and identification strategies
Day #2
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| 09:30 - 11:00 | Peptide identification using search engines |
| 11:00 - 11:30 | Coffee Break |
| 11:30 - 12:30 | Practical: searching mass spectral data (OMSSA, XTandem) |
| 12:30 - 14:00 | Lunch Break |
| 14:00 - 16:00 | Protein databases and decoy searches |
| 16:00 - 16:30 | Tea Break |
| 16:30 - 18:00 | Practical: creating and using custom databases |
| Wed, Sept 15th | Post-identification validation and protein inference
Day #3
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| 09:30 - 11:00 | Post-identification validation strategies |
| 11:00 - 11:30 | Coffee Break |
| 11:30 - 12:30 | Practical: evaluating peptide identifications |
| 12:30 - 14:00 | Lunch Break |
| 14:00 - 16:00 | Protein inference - challenges and issues |
| 16:00 - 16:30 | Tea Break |
| 16:30 - 18:00 | Practical: protein inference analysis (MassSieve) |
| Thu, Sept 16th | Interpreting quantitative proteomics data
Day #4
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| 09:30 - 11:00 | Quantitative proteomics data processing |
| 11:00 - 11:30 | Coffee Break |
| 11:30 - 12:30 | Practical: analysing quantitative proteomics data |
| 12:30 - 14:00 | Lunch Break |
| 14:00 - 16:00 | experimental design and results analysis |
| 16:00 - 16:30 | Tea Break |
| 16:30 - 18:00 | Practical: designing experiments and applying statistics |
| Fri, Sept 17th | Functional analysis and dissemination of data
Day 5
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| 09:30 - 11:00 | Functional analysis of proteomics data |
| 11:00 - 11:30 | Coffee Break |
| 11:30 - 12:30 | Practical: annotating a protein list (BioMart / GO / iceLogo) |
| 12:30 - 14:00 | Lunch Break |
| 14:00 - 16:00 | Dissemination of proteomics data |
| 16:00 - 16:30 | Tea Break |
| 16:30 - 18:00 | Practical: preparing a data submission using PRIDE Converter |
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Instituto Gulbenkian de Ciência, Apartado 14, 2781-901 Oeiras, Portugal Last updated: July 28th 2010 |