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WESRDA13
GTPB: Whole Exome Sequencing and RNA-seq data analysisCourse Timetable (provisional) |
| WESRDA13 | Whole Exome Sequencing and RNA-seq data analysis |
| Tue, Oct 8th |
Day #1
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| 09:30 - 10:00 | Welcome and Introductions |
| 10:00 - 11:00 | Introduction to NGS Technologies for Genomic Studies |
| 11:00 - 11:30 | Coffee Break |
| 11:30 - 12:30 | Quality Control for NGS Raw Data (FASTQ) and Data Preprocessing |
| 12:30 - 14:00 | Lunch Break |
| 14:00 - 16:00 | Mapping NGS Reads for Exome and Transcriptomics Studies I |
| 16:00 - 16:30 | Tea Break |
| 16:30 - 18:00 | Mapping NGS Reads for Exome and Transcriptomics Studies II |
| Wed, Oct 9th |
Day #2
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| 09:30 - 11:00 | Mapping Quality Control (BAM) and Exome Capture Evaluation |
| 11:00 - 11:30 | Coffee Break |
| 11:30 - 12:30 | Visualization of Mapped Reads |
| 12:30 - 14:00 | Lunch Break |
| 14:00 - 16:00 | Variant Calling (SNPs & INDELs) and Variant Quality Control (VCF) |
| 16:00 - 16:30 | Tea Break |
| 16:30 - 18:00 | ariant Annotation |
| Thu, Oct 10th |
Day #3
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| 09:30 - 11:00 | Variant and Gene Prioritization for Exome Studies |
| 11:00 - 11:30 | Coffee Break |
| 11:30 - 12:30 | RNA-Seq Quantification and Isoforms Finding |
| 12:30 - 14:00 | Lunch Break |
| 14:00 - 16:00 | RNA-Seq Normalization and Differential Expression Analysis I |
| 16:00 - 16:30 | Tea Break |
| 16:30 - 18:00 | Differential Expression analysis II |
| Fri, Oct 11th |
Day #4
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| 09:30 - 11:00 | Cloud Computing Resources for NGS |
| 11:00 - 11:30 | Coffee Break |
| 11:30 - 12:30 | Guided Exercise, putting everything together |
| 12:30 - 14:00 | Lunch Break |
| 14:00 - 16:00 | Practical Session with Sequence Data I |
| 16:00 - 16:30 | Tea Break |
| 16:30 - 18:00 | Practical Session with Sequence Data II |
| Course Homepage |
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Instituto Gulbenkian de Ciência, Apartado 14, 2781-901 Oeiras, Portugal |