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SMLMC15
Structural Modelling for Large Macromolecular ComplexesTimetable (provisional) |
| SMLMC15 | Structural Modelling for Large Macromolecular Complexes |
| Mon, Apr 27th |
Day #1
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| 09:30 - 11:00 |
Welcome and introductions An introduction to structure determination of protein and RNA structures. |
| 11:00 - 11:30 | Coffee Break |
| 11:30 - 12:30 | Prediction of protein and RNA tertiary structure. |
| 12:30 - 14:00 | Lunch Break |
| 14:00 - 16:00 | Prediction of RNA tertiary structure using homology modeling approach (ModeRNA in action). |
| 16:00 - 16:30 | Tea Break |
| 16:30 - 18:00 | Prediction of protein tertiary structure using homology modeling approach (Modeller and SWISS-Model). |
| Tue, Apr 28th |
Day #2
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| 09:30 - 11:00 |
Wrap-up of Day #1 (what have we done so far?) More practical examples on RNA and protein structure prediction and visualization. |
| 11:00 - 11:30 | Coffee Break |
| 11:30 - 12:30 | Structure determination of large macromolecular complexes. |
| 12:30 - 14:00 | Lunch Break |
| 14:00 - 16:00 | Working with large macromolecular complexes: tools and databases. |
| 16:00 - 16:30 | Tea Break |
| 16:30 - 18:00 | Hands-on practical familiarization with open source visualization software, UCSF Chimera. Working with electron density maps. |
| Wed, Apr 29th |
Day #3
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| 09:30 - 11:00 |
Wrap-up of Day #2 (what have we done so far?) The Hybrid Modelling approach |
| 11:00 - 11:30 | Coffee Break |
| 11:30 - 12:30 | Introduction to the PyRy3D software. |
| 12:30 - 14:00 | Lunch Break |
| 14:00 - 16:00 | Modelling of copper-nitrite reductase complex structure using experimental data from X-ray crystallography and electron microscopy. The PyRy3D GUI in action. |
| 16:00 - 16:30 | Tea Break |
| 16:30 - 18:00 | Analysis and visualization of copper-nitrite reductase complex structure models with PyRy3D GUI. |
| Thu, Apr 30th |
Day #4
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| 09:30 - 11:00 |
Wrap-up of Day #3 (what have we done so far?) Didactic exploration of case studies of successful analysis performed with integrative modelling approach. |
| 11:00 - 11:30 | Coffee Break |
| 11:30 - 12:30 | Using PyRy3D for structure prediction of RNA structure based on homology models and SAXS curves. |
| 12:30 - 14:00 | Lunch Break |
| 14:00 - 16:00 | Using PyRy3D for modeling of human polymarase II using cryo-EM map, crosslinking data, and crystal structures and homology models of the subunits. |
| 16:00 - 16:30 | Tea Break |
| 16:30 - 18:30 |
Analysis and visualization of human polimarase II models. Critical assessment. Final Wrap-up session. |
| Course Homepage |
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Instituto Gulbenkian de Ciência, Apartado 14, 2781-901 Oeiras, Portugal Last updated: Mar 25th 2015 |